Abrogation of Acute Inflammation in ICR Mice with Topical Administration of Philippine Stingless Bee (Tetragonula biroi Friese) Honey

Pharmacognosy Research,2023,15,2,258-266.DOI:10.5530/pres.15.2.028Published:February 2023Type:Original Article Authors:Mark Joseph Maranan Desamero, Mikaela Bernadette Andres Rabino, Maria Angelica Rimas Tayao, Mary Jasmin Cabillon Ang, Jussiaea Valente Bariuan, Roxanne Posilero Gapasin, Therese Marie Avena Collantes, Melissa Risos Rondina, Cherry Pambid Fernandez-Colorado, Cleofas Rodriguez Cervancia, and Amelita Celino Estacio Author(s) affiliations:Mark Desamero1,2,*, Rabino1, Tayao1, Ang1, Bariuan1,2, Gapasin1,2, Collantes3, Rondina4, Fernandez-Colorado3, Cervancia2,5, Estacio1,2 1Department of Basic Veterinary Sciences, College Medicine, University the Philippines Los Baños, Laguna, PHILIPPINES. 2UPLB Bee Program, 3Department Paraclinical 4Department Clinical 5Institute Biological Arts PHILIPPINES Abstract:Background: Honey, a sweet sugar-rich bee-derived product, has been widely recognized in realm Ayurveda for treatment various inflammatory conditions including bowel disease, asthma, arthritis, gastric ulcer. However, anti-inflammatory effect Philippine honey obtained from local stingless bees remains unexplored poorly investigated. Hence, this study delved on potential topically applied bee (PSH) using λ-Carrageenan-induced mouse model paw edema. Materials Methods: A total 30, male ICR mice six weeks age were randomly divided into following groups (n=10 per group) as follows: Distilled water-, 1% Diclofenac sodium-, Honey-treated group. All treatments administered immediately after Carrageenan injection then every 8 hr during 24-hr test duration. Results: Repeated topical application PSH significantly attenuated gross hind swelling observed at onset 6 post-induction (pi) until 24 pi with contrast to water-treated This corresponded histologically marked reduction dermal thickness, edema, leukocytic infiltration. Whereas, cytokine level, correlated significant suppression circulating blood levels pro-inflammatory cytokines, TNF-α, IL-1β, IL-6. Conclusion: Based these results, shows convincing activity which is partly mediated through regulation expression inflammation-associated cytokines. Keywords:Anti-inflammatory, IL-6, bees, TNF-α.View:PDF (1.75 MB)